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Research
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Grape Seed Extracts: |
Procyanidin
Oligomers selectively and intensively promote proliferation of
mouse hair epithelial cells in vitro and activate hair follicle
growth in vivo.
J Invest Dermatol 1999 Mar;112 (3):310-6
Takahashi T, Kamiya T, Hasegawa A, Yokoo Y
Tsukuba Research Laboratories, Kyowa Hakko Kogyo, Ibaraki,
Japan.
We have previously reported that proanthocyanidins extracted
from grape seeds possess growth-promoting activity toward murine
hair epithelial cells in vitro and stimulate anagen induction in
hair cycle progression in vivo. This report constitutes a
comparison of the growth-promoting activity of procyanidin
oligomers and the target cells of procyanidins in the skin.
Results show that procyanidin dimer and trimer exhibit higher
growth-promoting activity than the monomer. The maximum
growth-promoting activity for hair epithelial cells with
procyanidin B-2, an epicatechin dimer, reached about 300% (30
microM) relative to controls (= 100%) in a 5 d culture. Optimum
concentration of procyanidin C-1, an epicatechin trimer, was
lower than that of procyanidin B-2; the maximum growth-promoting
activity of procyanidin C-1 was about 220% (3 microM). No other
flavonoid compounds examined exhibit higher proliferative
activities than the procyanidins. In skin constituent cells,
only epithelial cells such as hair keratinocytes or epidermal
keratinocytes respond to procyanidin oligomers. Topical
application of 1% procyanidin oligomers on shaven C3H mice in
the telogen phase led to significant hair regeneration [procyanidin
B-2, 69.6% +/- 21.8% (mean +/- SD); procyanidin B-3, 80.9% +/-
13.0%; procyanidin C-1, 78.3% +/- 7.6%] on the basis of the
shaven area; application of vehicle only led to regeneration of
41.7% (SD = 16.3%). In this paper, we demonstrate the
hair-growing activity of procyanidin oligomers both in vitro and
in vivo, and their potential for use as agents to induce hair
growth.
PMID: 10084307, UI: 99181798 |
Procyanidin
Oligomers selectively and intensively promote proliferation of
mouse hair epithelial cells in vitro and activate hair follicle
growth in vivo.
J Invest Dermatol 1999 Mar;112 (3):310-6
Takahashi T, Kamiya T, Hasegawa A, Yokoo Y
Tsukuba Research Laboratories, Kyowa Hakko Kogyo, Ibaraki,
Japan.
We have previously reported that proanthocyanidins extracted
from grape seeds possess growth-promoting activity toward murine
hair epithelial cells in vitro and stimulate anagen induction in
hair cycle progression in vivo. This report constitutes a
comparison of the growth-promoting activity of procyanidin
oligomers and the target cells of procyanidins in the skin.
Results show that procyanidin dimer and trimer exhibit higher
growth-promoting activity than the monomer. The maximum
growth-promoting activity for hair epithelial cells with
procyanidin B-2, an epicatechin dimer, reached about 300% (30
microM) relative to controls (= 100%) in a 5 d culture. Optimum
concentration of procyanidin C-1, an epicatechin trimer, was
lower than that of procyanidin B-2; the maximum growth-promoting
activity of procyanidin C-1 was about 220% (3 microM). No other
flavonoid compounds examined exhibit higher proliferative
activities than the procyanidins. In skin constituent cells,
only epithelial cells such as hair keratinocytes or epidermal
keratinocytes respond to procyanidin oligomers. Topical
application of 1% procyanidin oligomers on shaven C3H mice in
the telogen phase led to significant hair regeneration [procyanidin
B-2, 69.6% +/- 21.8% (mean +/- SD); procyanidin B-3, 80.9% +/-
13.0%; procyanidin C-1, 78.3% +/- 7.6%] on the basis of the
shaven area; application of vehicle only led to regeneration of
41.7% (SD = 16.3%). In this paper, we demonstrate the
hair-growing activity of procyanidin oligomers both in vitro and
in vivo, and their potential for use as agents to induce hair
growth.
PMID: 10084307, UI: 99181798 |
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